Prohibitin antibody (AA 171-272)

Catalog No. ABIN686692

This Rabbit Polyclonal antibody specifically detects Prohibitin in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for Prohibitin antibody (AA 171-272) (ABIN686692)

Target: Prohibitin (PHB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Prohibitin Antibody

Binding Specificity: AA 171-272

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Prohibitin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Prohibitin antibody (ABIN686692)

Wang, Shi, He, Lu, Li, Chen: "Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus." in: Scientific reports, Vol. 6, pp. 34308, (2016) (PubMed).

Xing, Yuan, Xu, Gu, Liang, Lu: "Proteomic identification of mitochondrial targets involved in andrographolide sodium bisulfite-induced nephrotoxicity in a rat model." in: Environmental toxicology and pharmacology, Vol. 40, Issue 2, pp. 592-9, (2015) (PubMed).

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: Prohibitin

Background:

Synonyms: PHB1, HEL-215, HEL-S-54e, Prohibitin, PHB

Background: Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

Gene ID: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling