RAP1A antibody (AA 101-184) (HRP)

Catalog No. ABIN687481

This Rabbit Polyclonal antibody specifically detects RAP1A in WB, ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for RAP1A antibody (AA 101-184) (HRP) (ABIN687481)

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAP1A antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RAP1A Antibody (HRP)

Binding Specificity: AA 101-184

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RAP1A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RAP1A

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Alternative Name: RAP1A

Background:

Synonyms: RAP1, C21KG, G-22K, KREV1, KREV-1, SMGP21, Ras-related protein Rap-1A, GTP-binding protein smg p21A, Ras-related protein Krev-1, RAP1A

Background: Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.

Gene ID: 5906

UniProt: P62834

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor