POLR2A/RPB1 antibody (pSer5) (Biotin)

Catalog No. ABIN6874987

The Rabbit Monoclonal anti-POLR2A/RPB1 antibody has been validated for WB, IF, IHC, FACS and ICC. It is suitable to detect POLR2A/RPB1 in samples from Human, Mouse and Rat.

Quick Overview for POLR2A/RPB1 antibody (pSer5) (Biotin) (ABIN6874987)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This POLR2A/RPB1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: JM51-21

Product Details anti-POLR2A/RPB1 Antibody (Biotin)

Binding Specificity: pSer5

Purpose: Conjugated POLR2A (Phospho-S5) Rabbit mAb

Purification: Protein A affinity purified

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser5 of human POLR2A.

Application Details

Application Notes: Biotin conjugated: Working with enzyme-conjugated streptavidin. ELISA: 1: 200 - 1: 1,000; Western Blot: 1: 200 - 1: 1,000; IHC/ICC: 1: 100 - 1: 500; Flow Cytometry: 1: 50 - 1: 250; IF: 1: 100 - 1: 500.

Restrictions: For Research Use only

Handling

Buffer: Liquid in PBS, 0.5 % BSA, 40 % Glycerol, 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light!

Storage: 4 °C

Storage Comment: Store at 4°C in dark for 6 months

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A

Background: DNA directed RNA polymerase II A antibody DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit antibody DNA-directed RNA polymerase II subunit A antibody DNA-directed RNA polymerase II subunit RPB1 antibody DNA-directed RNA polymerase III largest subunit antibody hRPB220 antibody hsRPB1 antibody POLR2 antibody Polr2a antibody POLRA antibody Polymerase (RNA) II (DNA directed) polypeptide A 220 kDa antibody Polymerase (RNA) II (DNA directed) polypeptide A antibody RNA polymerase II subunit B1 antibody RNA-directed RNA polymerase II subunit RPB1 antibody RPB1 antibody RPB1_HUMAN antibody RPBh1 antibody RpIILS antibody RPO2 antibody RPOL2 antibody

Molecular Weight: Predicted band size: 217 kDa

Gene ID: 5430

UniProt: P24928

Pathways: Regulatory RNA Pathways