CEACAM1/5 antibody (CF®488A)

Catalog No. ABIN6933700

Product Details anti-CEACAM1/5 Antibody (CF®488A)

Target: CEACAM1/5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM1/5 antibody is conjugated to CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Specificity: This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Cross-Reactivity (Details): Human,

Purification: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules.

Immunogen: Recombinant full-length human CEA protein

Clone: C66-1009

Isotype: IgG2a

Application Details

Application Notes:

Positive Control: MCF7 or 293T cells. Colon carcinoma.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for CEACAM1/5

Target: CEACAM1/5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5))

Alternative Name: CEACAM1 & CEACAM5 (CEACAM1/5 Products)

Synonyms: CD66e antibody, CEA antibody, carcinoembryonic antigen related cell adhesion molecule 5 antibody, CEACAM5 antibody

Background: Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1),Carcinoembryonic Antigen (CEA) / CD66
Cellular localisation: Cytoplasmic and luminal surface

Molecular Weight: 80-200kDa

Gene ID: 1048, 634, 709196

UniProt: P06731