EpCAM antibody

Catalog No. ABIN6933744

The Mouse Monoclonal anti-EpCAM antibody has been validated for WB, FACS, IF, IHC (p), IP and IHC (fro). It is suitable to detect EpCAM in samples from Rat.

Quick Overview for EpCAM antibody (ABIN6933744)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: SPM534

Product Details anti-EpCAM Antibody

Specificity: Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.

No Cross-Reactivity: Human

Immunogen: Rat small intestine cells

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Rat small intestine or colon.

Known Application: Flow Cytometry (5-10 µL/million cells), Immunofluorescence (1:50-1:100), Western Blot (1:100-1:200), Immunoprecipitation (5-10 µL/500 μg protein lysate),Immunohistochemistry (Frozen & formalin-fixed) (1:100-1:200 for 30 min at RT)(Formalin/paraffin tissues REQUIRE digestion of tissue sections with pepsin at 1 mg/mL Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: Bioreactor Concentrate with 0.05 % Azide. Contact us if you require it in a different format.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: Ep-CAM / CD326

Molecular Weight: 40-43kDa

Gene ID: 171577

UniProt: O55159