IGHA1 & IGHA2 antibody (CF®488A)

Catalog No. ABIN6933799

Product Details

Target: IGHA1 & IGHA2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®488A

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Specificity: This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Alpha heavy chain. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins.IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Purification: Purified by Protein A/G

Immunogen: Purified human alpha heavy chain

Clone: IA761

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Daudi, 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Flow Cytometry (1-2 µL/million cells), Immunofluorescence (0.5-1.0 μg/mL 30 min RT),Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL 30 min RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: IGHA1 & IGHA2

Molecular Weight: 50-75kDa

Gene ID: 3493, 3494

UniProt: P01876, P01877