IGHM antibody (CF®488A)

Catalog No. ABIN6934381

This anti-IGHM antibody is a Mouse Monoclonal antibody detecting IGHM in IHC, WB, StM, FACS and IF. Suitable for Human.

Quick Overview for IGHM antibody (CF®488A) (ABIN6934381)

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHM antibody is conjugated to CF®488A

Application: Immunohistochemistry (IHC), Western Blotting (WB), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: IM373

Product Details anti-IGHM Antibody (CF®488A)

Specificity: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length heavy chain of human IgM

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IGHM

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Alternative Name: IGHM

Molecular Weight: 50-75kDa

Gene ID: 3507

UniProt: P01871, P20769