Nucleolin antibody (CF®488A)

Catalog No. ABIN6934519

This anti-Nucleolin antibody is a Mouse Monoclonal antibody detecting Nucleolin in IF, FACS, ISt, StM and T. Suitable for Human.

Quick Overview for Nucleolin antibody (CF®488A) (ABIN6934519)

Target: Nucleolin (NCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is conjugated to CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM), Test (T)

Clone: NCL-902

Product Details anti-Nucleolin Antibody (CF®488A)

Specificity: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

No Cross-Reactivity: Cow (Bovine), Mouse (Murine), Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: Recombinant human NCL protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Breast Cancer.

Known Application: Flow Cytometry (5 µL per test per one million cells in 0.1 mL or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: NCL

Molecular Weight: 76kDa

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization