Vimentin antibody (CF®488A)

Catalog No. ABIN6934573

This anti-Vimentin antibody is a Mouse Monoclonal antibody detecting Vimentin in IF, FACS and T. Suitable for Human, Pig, Cow, Chicken, Dog, Cat and Goat.

Quick Overview for Vimentin antibody (CF®488A) (ABIN6934573)

Target: Vimentin (VIM)

Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is conjugated to CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Test (T)

Clone: VM452

Product Details anti-Vimentin Antibody (CF®488A)

Specificity: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: Recombinant human vimentin protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Jurkat cells, Sarcomas, Melanomas.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: VIM

Molecular Weight: 57-60kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis