Cytokeratin 5 antibody (AA 318-491)

Catalog No. ABIN6934855

This anti-Cytokeratin 5 antibody is a Mouse Monoclonal antibody detecting Cytokeratin 5 in IHC (f). Suitable for Human.

Quick Overview for Cytokeratin 5 antibody (AA 318-491) (ABIN6934855)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: KRT5-2080

Product Details anti-Cytokeratin 5 Antibody

Binding Specificity: AA 318-491

Specificity: This MAb recognizes a protein of 58 kDa, which is identified as Cytokeratin 5 (KRT5). This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, myoepithelia, and mesothelium.Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express.Anti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5.

Cross-Reactivity (Details): Human.

Immunogen: Recombinant human Cytokeratin 5 (KRT5) protein fragment (around aa 318-491) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or HeLa cells. Tonsil, Esophagus, Cervix or Urinary Bladder. Basal Cell or Squamous Cell Carcinoma.

Restrictions: For Research Use only

Handling

Buffer: Tissue culture supernatant with 0.05% Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: KRT5

Background: 58 kDa Cytokeratin, CK5, Cytokeratin-5, DDD1, Epidermolysis Bullosa Simplex 2 (EBS2), Keratin 5, Keratin, Type II Cytoskeletal 5, Keratin-5, KRT5, Type-II Cytoskeletal 5, Type-II keratin Kb5,Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 58kDa

Gene ID: 3852, 433845

UniProt: P13647