ABO antibody

Catalog No. ABIN6936339

The Mouse Monoclonal anti-ABO antibody has been validated for AGG, H and IHC (f). It is suitable to detect ABO in samples from Human.

Quick Overview for ABO antibody (ABIN6936339)

Target: ABO (Histo-blood group A transferase (ABO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ABO antibody is un-conjugated

Application: Agglutination (AGG), Haemagglutination (H), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: HEB-20

Product Details anti-ABO Antibody

Specificity: The antibody HEB-20 reacts with human blood group B. The specificity of the antibody HEB-20 was confirmed by comparison of specificity and reactivity to standard reagent using 5.000 samples of blood. The MAb HEB-20 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. This MAb is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Mixture of erythrocytes of group B and glyco protein fraction isolated from saliva of secretors with blood group B

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Agglutination, Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: KG1 cells. Human colorectal carcinoma tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ABO

Target: ABO (Histo-blood group A transferase (ABO))

Alternative Name: ABO

Background: A transferase, ABO, B transferase, CD173, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT,Blood Group Antigen B (CD173)
Cellular localisation: Cell Surface

Molecular Weight: Multiple

Gene ID: 28, 654423

UniProt: P16442