ACTN2 antibody (AA 557-692)

Catalog No. ABIN6936358

This anti-ACTN2 antibody is a Mouse Monoclonal antibody detecting ACTN2 in IHC (f). Suitable for Human.

Quick Overview for ACTN2 antibody (AA 557-692) (ABIN6936358)

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTN2 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ACTN2-3291

Product Details anti-ACTN2 Antibody

Binding Specificity: AA 557-692

Specificity: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinantfragment (aa557-692) ofhuman ACTN2 protein(exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Skeletal or Cardiac Muscle.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ACTN2

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Alternative Name: ACTN2

Background: Actin binding protein, Alpha actinin skeletal muscle, isoform 2, CMD1AA, F-actin cross-linking protein,Sarcomeric Actinin Alpha 2 / ACTN2
Cellular localisation: Cytoplasmic

Molecular Weight: 103kDa

Gene ID: 88, 498178

UniProt: P35609

Pathways: Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity