alpha Fetoprotein antibody

Catalog No. ABIN6936365

This Mouse Monoclonal antibody specifically detects alpha Fetoprotein in IHC. It exhibits reactivity toward Human, Pig, Dog and Monkey.

Quick Overview for alpha Fetoprotein antibody (ABIN6936365)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human, Pig, Dog, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: C3

Product Details anti-alpha Fetoprotein Antibody

Purpose: AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody

Specificity: It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

No Cross-Reactivity: Cow (Bovine), Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Human. Monkey. Dog. Pig. Does not react with Cow, Mouse or Rat.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Alpha feto protein (AFP) Purified from serum of a hepatoma patient

Isotype: IgG2a, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP

Background:

Alpha fetoglobulin, FETA, HPAFP

Hep-G2 cells. Fetal liver or hepatocellular carcinoma.

Molecular Weight: 70kDa

Gene ID: 174, 518808

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process