alpha Fetoprotein antibody

Catalog No. ABIN6936367

The Mouse Monoclonal anti-alpha Fetoprotein antibody (Clone C2-C3-MBS-12) (ABIN6936367) specifically detects alpha Fetoprotein in WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for alpha Fetoprotein antibody (ABIN6936367)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: C2-C3-MBS-12

Product Details anti-alpha Fetoprotein Antibody

Purpose: AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody

Specificity: It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Alpha feto protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2 cells. Fetal liver or hepatocellular carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP

Background:

Alpha fetoglobulin, FETA, HPAFP

HepG2 cells. Fetal liver or hepatocellular carcinoma.

Molecular Weight: 70kDa

Gene ID: 174, 518808

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process