alpha Fetoprotein antibody

Catalog No. ABIN6936368

This Mouse Monoclonal antibody specifically detects alpha Fetoprotein in WB, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for alpha Fetoprotein antibody (ABIN6936368)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: MBS-12

Product Details anti-alpha Fetoprotein Antibody

Purpose: AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody

Specificity: It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. The yolk sac and the liver produce AFP during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spinal bifida and anencephaly.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human Alpha feto protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP

Background:

Alpha fetoglobulin, FETA, HPAFP

Hep G2 cells. Fetal liver or hepatocellular carcinoma.

Molecular Weight: 70kDa

Gene ID: 174, 518808

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process