Recombinant PLAP antibody

Catalog No. ABIN6936401

This Rabbit Monoclonal antibody specifically detects PLAP in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant PLAP antibody (ABIN6936401)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PLAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ALPP-2899R

Product Details anti-PLAP Antibody

Purpose: Recombinant Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Antibody

Specificity: Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant full-length human ALPP protein

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues),Optimal dilution for a specific application should be determined.

Positive_Control: JEG-3 or HepG2 cells. Placenta or seminoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: ALPP

Background:

Alkaline phosphatase placental type, Alkaline phosphatase Regan Isozyme, ALP, Alp1, ALPP, Germ-cell alkaline phosphatase, nagao Isozyme, PALP, Placental alkaline phosphatase 1, placental heat-stable alkaline phosphatase, PLAP-1, PLAP1

JEG-3 or HepG2 cells. Placenta or seminoma.

Molecular Weight: 70kDa

Gene ID: 250, 284255

UniProt: P05187