ATG5 antibody (AA 1-119)

Catalog No. ABIN6936433

This anti-ATG5 antibody is a Mouse Monoclonal antibody detecting ATG5 in WB, ELISA, Coat and IHC (f). Suitable for Human.

Quick Overview for ATG5 antibody (AA 1-119) (ABIN6936433)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ATG5-2553

Product Details anti-ATG5 Antibody

Binding Specificity: AA 1-119

Specificity: The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy, a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K562, U-87, A431, THP-1, PANC-1, Raji or HeLa cells. Ovary, Endometrium, Colon or Duodenum.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background: APG5 autophagy 5 like (APG5L), Apoptosis-specific protein (ASP), ATG5, Autophagy related 5, hAPG5,ATG5 (Autophagy Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 32kDa

Gene ID: 9474, 486063

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy