Recombinant Aurora Kinase B antibody (AA 89-251)

Catalog No. ABIN6936444

The Mouse Monoclonal anti-Aurora Kinase B antibody has been validated for IHC (f). It is suitable to detect Aurora Kinase B in samples from Human.

Quick Overview for Recombinant Aurora Kinase B antibody (AA 89-251) (ABIN6936444)

Target: Aurora Kinase B (AURKB)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RAURKB-1592

Product Details anti-Aurora Kinase B Antibody

Binding Specificity: AA 89-251

Specificity: Recognizes a protein of 39 kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Tonsil, Placenta, Hodgkin s Lymphoma or Colon Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: AURKB

Background: AIK2, AIM-1, ARK-2, AurB, AURKB, Aurora-1, Aurora and Ipl1 like midbody associated protein 1, Aurora kinase B, Aurora-B, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, IPL1, Protein phosphatase 1 regulatory subunit 48 (PPP1R48), Serine/threonine-protein kinase 12, Serine/threonine-protein kinase aurora-B, STK1, STK12, STK5,Aurora B (Proliferation Marker)
Cellular localisation: Nuclear and Cytoplasmic

Molecular Weight: 39kDa

Gene ID: 9212, 442658

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades