beta-2 Microglobulin antibody

Catalog No. ABIN6936450

The Mouse Monoclonal anti-beta-2 Microglobulin antibody has been validated for WB, FACS, IF and IHC (fro). It is suitable to detect beta-2 Microglobulin in samples from Human.

Quick Overview for beta-2 Microglobulin antibody (ABIN6936450)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BBM-1

Product Details anti-beta-2 Microglobulin Antibody

Specificity: Recognizes a protein of 12 kDa, identified as microglobulin. Major histocompatibility complex (MHC) class 1 Molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 Molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 Molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Cross-Reactivity (Details): Human. Non-Human primates.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: MOLT-4 human T cell line

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-4 μg/mL), Immunohistochemistry (Frozen) (1-2 μg/mL for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Positive_Control: THP-1, Raji, HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: B2M

Background: B2M, Beta 2 microglobin, Beta 2 microglobulin, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, Hdcma22p,Beta-2 Microglobulin (Renal Failure & Tumor Marker)
Cellular localisation: Cell Surface and Extracellular (Secreted)

Molecular Weight: 12kDa

Gene ID: 567, 534255

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process