Recombinant BCL6 antibody (AA 256-389)

Catalog No. ABIN6936504

The Rabbit Monoclonal anti-BCL6 antibody has been validated for IHC (f). It is suitable to detect BCL6 in samples from Human.

Quick Overview for Recombinant BCL6 antibody (AA 256-389) (ABIN6936504)

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BCL6 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: BCL6-2497R

Product Details anti-BCL6 Antibody

Binding Specificity: AA 256-389

Specificity: Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ('LH') cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

Cross-Reactivity (Details): Human. Dog

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human bcl-6 protein fragment (aa256-389) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Raji or Ramos cells. Tonsil or Hodgkin's lymphoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Alternative Name: BCL6

Background: B-cell lymphoma 5 protein, B-Cell Lymphoma 6 Protein, BCL5, BCL6, BCL6A, cys his2 zinc finger transcription factor, Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3), Zinc finger and BTB domain-containing protein 27 (ZBTB27), Zinc Finger Protein 51 (ZNF51), zinc finger transcription factor BCL6S,Bcl-6 (Follicular Lymphoma Marker)
Cellular localisation: Nuclear

Molecular Weight: 95kDa

Gene ID: 604, 478588

UniProt: P41182

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus