BMI1 antibody (AA 142-326)

Catalog No. ABIN6936510

The Mouse Monoclonal anti-BMI1 antibody has been validated for WB, IF and IHC (f). It is suitable to detect BMI1 in samples from Human.

Quick Overview for BMI1 antibody (AA 142-326) (ABIN6936510)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: BMI1-2823

Product Details anti-BMI1 Antibody

Binding Specificity: AA 142-326

Specificity: The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.

Cross-Reactivity (Details): Human. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 142-326) of human BMI1 protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells. Colon Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1

Background: B lymphoma Mo MLV insertion region 1 homolog, BMI1, BMI1 polycomb ring finger oncogene, FLVI2/BMI1, Oncogene BMI1, PCGF4, Polycomb complex protein BMI-1, Polycomb group ring finger 4, RING finger protein 51, RNF51,BMI1 (Oncogene and Stem Cell Marker)
Cellular localisation: Nuclear

Molecular Weight: 41kDa

Gene ID: 648, 380403

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy