Recombinant BrdU antibody

Catalog No. ABIN6936522

The Mouse Monoclonal anti-BrdU antibody is suitable to detect BrdU in samples from Various Species. It has been validated for IHC.

Quick Overview for Recombinant BrdU antibody (ABIN6936522)

Target: BrdU (Bromodeoxyuridine (BrdU))

Antibody Type: Recombinant Antibody

Reactivity: Various Species

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RBRD-3

Product Details anti-BrdU Antibody

Purpose: Recombinant Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody

Specificity: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Bromodeoxyuridine (BrdU) conjugated to BSA

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: Bromodeoxyuridine (BrdU) (Proliferation Marker)

Target Type: Chemical

Background:

Bromodeoxyuridine, BUdr

Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Molecular Weight: Depends on the target