MUC16 antibody

Catalog No. ABIN6936546

The Mouse Monoclonal anti-MUC16 antibody has been validated for IHC (f). It is suitable to detect MUC16 in samples from Human.

Quick Overview for MUC16 antibody (ABIN6936546)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC16 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: MUC16-1860

Product Details anti-MUC16 Antibody

Specificity: The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs). Membrane-associated and secretory Mucins are high molecular weight glycoproteins of the glycocalyx (polysaccharide biofilm) that protects mucosal epithelium from particulate matter and microorganisms. Epithelial Mucins are large, secreted and cell surface glycoproteins crucial for adhesion modulation, signaling and epithelial cell protection. The number of repeats is highly polymorphic and varies among different alleles. The Mucin family consists of Mucins 1-4, Mucin 5 (AC and B), Mucins 6-8, Mucins 11-13 and Mucins 15-17. The Mucin 16 protein (also commonly referred to as CA125), encoded for by the gene MUC16, is a very high molecular weight tumor antigen consisting of three domains: a carboxy terminal domain, an extracellular domain and an amino terminal domain. Mucin 16, an ovarian cancer-associated antigen, is used as a marker to monitor the progress of epithelial ovarian cancer. It is a hydrophilic membrane-associated protein that may be involved in vitamin A functions.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Full length native MUC16 protein Purified from human ovarian carcinoma

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MDA-MB-468 cells. Ovarian Cancer.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MUC16

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: CA125

Background: CA125, Cancer antigen 125, MUC16, Mucin16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125,MUC16 / CA125 (Ovarian Carcinoma Marker)
Cellular localisation: Cell Surface and Secreted

Molecular Weight: >2,000kDa

Gene ID: 94025, 432676

UniProt: Q8WXI7