CA8 antibody

Catalog No. ABIN6936553

This anti-CA8 antibody is a Mouse Monoclonal antibody detecting CA8 in WB and IHC (f). Suitable for Human.

Quick Overview for CA8 antibody (ABIN6936553)

Target: CA8 (Carbonic Anhydrase VIII (CA8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CPTC-CA8-2

Product Details anti-CA8 Antibody

Specificity: The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in CA8 gene causes neuropathology, such as ataxia, mild mental retardation and the predisposition to quadrupedal gait. It is also associated with the development of colorectal and lung cancers. Additionally, it is upregulated in various cancers.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CA8 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human cerebellum. Selective expression in purkinje cells. HEK-293 or K-562 cell lines.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CA8

Target: CA8 (Carbonic Anhydrase VIII (CA8))

Alternative Name: CA8

Background: CA-VIII, CALS, CARP, MGC120502, MGC99509,Carbonic Anhydrase VIII
Cellular localisation: Cytoplasm. Cell surface.

Molecular Weight: 32.97kDa

Gene ID: 767, 654388

UniProt: P35219