CA8 antibody

Catalog No. ABIN6940916

The Mouse Monoclonal anti-CA8 antibody is suitable to detect CA8 in samples from Human. It has been validated for WB and IHC.

Quick Overview for CA8 antibody (ABIN6940916)

Target: CA8 (Carbonic Anhydrase VIII (CA8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CPTC-CA8-2

Product Details anti-CA8 Antibody

Purpose: Carbonic Anhydrase VIII Antibody

Specificity: The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in CA8 gene causes neuropathology, such as ataxia, mild mental retardation and the predisposition to quadrupedal gait. It is also associated with the development of colorectal and lung cancers. Additionally, it is upregulated in various cancers.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human CA8 protein

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: Human cerebellum. Selective expression in purkinje cells. HEK-293 and K-562 cell lines.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CA8

Target: CA8 (Carbonic Anhydrase VIII (CA8))

Alternative Name: CA8

Background:

CA-VIII, CALS, CARP, MGC120502, MGC99509

Human cerebellum. Selective expression in Purkinje cells. HEK293 or K-562 cell lines.

Molecular Weight: 32.97kDa

Gene ID: 767, 654388

UniProt: P35219