Calpastatin antibody

Catalog No. ABIN6936579

The Mouse Monoclonal anti-Calpastatin antibody has been validated for ELISA, Coat and IHC (f). It is suitable to detect Calpastatin in samples from Human.

Quick Overview for Calpastatin antibody (ABIN6936579)

Target: Calpastatin (CAST)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calpastatin antibody is un-conjugated

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CAST-1550

Product Details anti-Calpastatin Antibody

Specificity: Calpastatin is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpastatin, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpastatin co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CAST protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Esophagus or Small Intestine.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Calpastatin

Target: Calpastatin (CAST)

Alternative Name: CAST

Background: BS 17, Calpain inhibitor, Calpastatin, Cast, Heart type calpastatin, Sperm BS 17 component,Calpastatin
Cellular localisation: Cytoplasm and Cell Surface

Molecular Weight: 126kDa

Gene ID: 831, 436186

UniProt: P20810