Cyclin B1 antibody

Catalog No. ABIN6936584

This Mouse Monoclonal antibody specifically detects Cyclin B1 in IF, FACS, IP and BP. It exhibits reactivity toward Hamster.

Quick Overview for Cyclin B1 antibody (ABIN6936584)

Target: Cyclin B1 (CCNB1)

Reactivity: Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP)

Clone: V92-1

Product Details anti-Cyclin B1 Antibody

Specificity: It recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Cross-Reactivity (Details): Human. Mouse. Hamster.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Hamster Cyclin B1 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein) (precipitates active CDK1/cyclin B1 complexes), Optimal dilution for a specific application should be determined.

Positive_Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB1

Background: CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1,Cyclin B1 (G2- & M-phase Cyclin)
Cellular localisation: Nuclear

Molecular Weight: 55-62kDa

Gene ID: 891, 23960

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase