DC-SIGN/CD209 antibody

Catalog No. ABIN6936638

This Mouse Monoclonal antibody specifically detects DC-SIGN/CD209 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for DC-SIGN/CD209 antibody (ABIN6936638)

Target: DC-SIGN/CD209 (CD209) (CD209)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DC-SIGN/CD209 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C209-1781

Product Details anti-DC-SIGN/CD209 Antibody

Purpose: CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) Antibody

Specificity: DC-SIGN is a transmembrane receptor that is expressed on the surface of dendritic cells and macrophages. It is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization, which allows the receptor to bind multivalent ligands with high avidity.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human CD209 protein fragment (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA Buffer, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: THP-1 or HeLa cells (IF/FACS). Human liver or spleen lysate (WB). Human small intestine (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for DC-SIGN/CD209

Target: DC-SIGN/CD209 (CD209) (CD209)

Alternative Name: CD209

Background:

CD209, CDSIGN, CIRE, CLEC4L, DC-SIGN, DC-SIGN1, DCSIGN, Dendritic cell-specific ICAM-3 Grabbing Non-integrin 1, HIV GP120 Binding Protein, SIGN-R1, SIGNR5

THP-1 or HeLa cells (IF/FACS). Human liver or spleen lysate (WB). Human small intestine (IHC).

Molecular Weight: 45kDa

Gene ID: 30835, 278694

UniProt: Q9NNX6