CD27 antibody

Catalog No. ABIN6936648

The Mouse Monoclonal anti-CD27 antibody is suitable to detect CD27 in samples from Human. It has been validated for FACS, ELISA, IF, IHC (f) and Coat.

Quick Overview for CD27 antibody (ABIN6936648)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD27 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Clone: LPFS2-1611

Product Details anti-CD27 Antibody

Specificity: Recognizes a protein of a disulfide-linked 120 kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CD27 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Ramos cells. Human peripheral blood lymphocytes. Ramos and lymph node cell lysates. Human tonsil and stomach tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background: LPFS2, S152, T cell activation antigen S152, T-cell activation antigen CD27, T14, TNFRSF7, TNFSF7, Tp55, Tumor necrosis factor receptor superfamily member 7,CD27 (Tumor Necrosis Factor Receptor Superfamily 7)
Cellular localisation: Cell Surface

Molecular Weight: 120kDa

Gene ID: 939, 355307

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints