PD-L1 antibody (AA 39-191)

Catalog No. ABIN6936654

The Mouse Monoclonal anti-PD-L1 antibody is suitable to detect PD-L1 in samples from Human. It has been validated for FACS, ELISA and IF.

Quick Overview for PD-L1 antibody (AA 39-191) (ABIN6936654)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Clone: PDL1-2743

Product Details anti-PD-L1 Antibody

Binding Specificity: AA 39-191

Purpose: PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) Antibody

Specificity: PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human CD274 protein (around aa 39-191) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/mL),Immunofluorescence (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: Heart, Placenta, Spleen or Tonsil (IHC). Jurkat cells (IF/FACS).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274

Background:

B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1

Heart, Placenta, Spleen or Tonsil (IHC). Jurkat cells (IF/FACS).

Molecular Weight: 37-50kDa

Gene ID: 29126, 521989

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints