CD34 antibody

Catalog No. ABIN6936665

This Mouse Monoclonal antibody specifically detects CD34 in FACS, IF, ELISA and Coat. It exhibits reactivity toward Human.

Quick Overview for CD34 antibody (ABIN6936665)

Target: CD34

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat)

Clone: ICO-115

Product Details anti-CD34 Antibody

Specificity: This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Cross-Reactivity (Details): Human. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Blast cells of a chronic myeloid leukemia patient

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: KG-1 cells. Human tonsil or angiosarcoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin,CD34 (Hematopoietic Stem Cell & Endothelial Marker)
Cellular localisation: Cell surface

Molecular Weight: 90-110kDa

Gene ID: 947, 374990

UniProt: P28906