CD34 antibody

Catalog No. ABIN6936667

This anti-CD34 antibody is a Mouse Monoclonal antibody detecting CD34 in FACS, IF and IHC (f). Suitable for Human.

Quick Overview for CD34 antibody (ABIN6936667)

Target: CD34

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SPM123

Product Details anti-CD34 Antibody

Specificity: This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intothree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain.CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi's sarcoma, but with a lower specificity.

No Cross-Reactivity: Cow (Bovine), Dog (Canine), Rat (Rattus), Sheep (Ovine)

Cross-Reactivity (Details): Human. Cynomolgus Monkey. Rhesus Monkey. Does not react with Rat, Sheep, Cow or Dog.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Detergent solubilized vesicular suspension prepared from human term placenta

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: KG-1 cells. Human tonsil or angiosarcoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin,CD34 (Hematopoietic Stem Cell & Endothelial Marker)
Cellular localisation: Cell surface

Molecular Weight: 90-110kDa

Gene ID: 947, 374990

UniProt: P28906