CD3 epsilon antibody (Cytoplasmic Domain)

Catalog No. ABIN6936685

The Mouse Monoclonal anti-CD3 epsilon antibody (Clone PC3-188A) (ABIN6936685) specifically detects CD3 epsilon in FACS, WB and IF. The antibody is reactive with Human, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig and Chicken samples.

Quick Overview for CD3 epsilon antibody (Cytoplasmic Domain) (ABIN6936685)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Clone: PC3-188A

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 156-168, Cytoplasmic Domain

Purpose: CD3e (T-Cell Marker) Antibody

Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Cross-Reactivity (Details): Human, Rat, Guinea pig, Rabbit, Pig, Horse, Cow and Chicken. Others not known.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3- chain

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Western Blotting (1-2 μg/mL for 60 minutes at RT),Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cells. Tonsil and lymph node

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background:

CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex

Jurkat cells. Tonsil and lymph node.

Molecular Weight: 20kDa

Gene ID: 916, 3003

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway