CD40 antibody

Catalog No. ABIN6936711

The Rat Monoclonal anti-CD40 antibody is suitable to detect CD40 in samples from Mouse. It has been validated for FACS, Func and IF.

Quick Overview for CD40 antibody (ABIN6936711)

Target: CD40

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD40 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func), Immunofluorescence (IF)

Clone: FGK45-5

Product Details anti-CD40 Antibody

Purpose: CD40, Mouse Antibody

Specificity: Upon binding to its ligand CD154, CD40 acts as a costimulatory molecule for the activation of B cells, dendritic cells, monocytes, and other APCs. CD40 plays roles in B cell activation, differentiation, proliferation and Ig isotype switching as well as dendritic cell maturation. FGK4.5 can be used for blocking of CD40/CD154 interaction and forin vitroandin vivoactivation of CD40 expressing APCs. This MAb is shown to indirectly activate natural killer (NK) cells, producing significant antitumor and anti-metastatic effects. Effective in boosting immune responses against infectious agents and can potentially be used to treat chronic autoimmune inflammatory processes.

No Cross-Reactivity: Human

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified recombinant Mouse CD40 fusion protein

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: MPBMC's. Spleen, Thymus or Lymph Node.

Known Application: Functional Studies (Order Antibody without BSA & Azide); Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background:

B cell surface antigen CD40, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TRAP, IMD3, IGM, Tnfrsf5, HIGM1, GP39, T-BAM

MPBMC's. Spleen, Thymus or Lymph Node.

Molecular Weight: 48kDa

Gene ID: 21939

UniProt: P27512

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints