Recombinant CD59 antibody

Catalog No. ABIN6936762

The Rabbit Monoclonal anti-CD59 antibody has been validated for IHC (f). It is suitable to detect CD59 in samples from Human.

Quick Overview for Recombinant CD59 antibody (ABIN6936762)

Target: CD59

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: MACIF-2867R

Product Details anti-CD59 Antibody

Specificity: Reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein (Workshop VI, Code N-L036). CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD59 functions.

No Cross-Reactivity: Baboon, Horse (Equine)

Cross-Reactivity (Details): Human. Does not react with Baboon or Horse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length CD59 protein

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat or Raji cells. Human lymphocytes. Human lymph node and tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background: 20 kDa homologous restriction factor (HRF20), Complement regulatory protein, Human leukocyte antigen MIC11, MAC-inhibitory protein (MACIP), Membrane attack complex inhibition factor (MACIF), Membrane inhibitor of reactive lysis, MIRL, MSK21, Protectin, T cell activating protein,CD59 / Complement Regulatory Protein / Protectin
Cellular localisation: Cell Surface

Molecular Weight: 20kDa

Gene ID: 966, 278573, 709466

UniProt: P13987

Pathways: Complement System