Recombinant CD68 antibody

Catalog No. ABIN6936786

The Mouse Monoclonal anti-CD68 antibody is suitable to detect CD68 in samples from Human. It has been validated for IHC, FACS, WB and IF.

Quick Overview for Recombinant CD68 antibody (ABIN6936786)

Target: CD68 (CD68 Molecule (CD68))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD68 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Clone: RLAMP4-824

Product Details anti-CD68 Antibody

Purpose: Recombinant CD68 (Macrophage Marker) Antibody

Specificity: This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human LAMP4 / CD68 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: U87MG, Tonsil, Lymph Node or Spleen.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68

Background:

GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1

lymph node or spleen., Tonsil, U87MG

Molecular Weight: 110kDa

Gene ID: 968, 647419

UniProt: P34810