CD7 antibody

Catalog No. ABIN6936795

The Mouse Monoclonal anti-CD7 antibody has been validated for FACS and IF. It is suitable to detect CD7 in samples from Human.

Quick Overview for CD7 antibody (ABIN6936795)

Target: CD7

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD7 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: B-F12

Product Details anti-CD7 Antibody

Specificity: Recognizes a protein of 40kDa, identified as CD7 (also known as gp40, Leu9). CD7 is a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Chronic Lymphoid Leukemia cells (CLL)

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, HUT-78, Molt-4, CEM cells or human peripheral blood lymphocytes. Lymph node and tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD7

Target: CD7

Alternative Name: CD7

Background: GP40, Leu9, p41, T-cell leukemia antigen, T-cell surface antigen Leu-9, Tp40, TP41,CD7 (T-Cell Leukemia Marker)
Cellular localisation: Cell Surface

Molecular Weight: 40kDa

Gene ID: 924, 186820

UniProt: P09564

Pathways: Cell-Cell Junction Organization