CD79b antibody (C-Term)

Catalog No. ABIN6936828

The Mouse Monoclonal anti-CD79b antibody has been validated for WB, IF and IHC (f). It is suitable to detect CD79b in samples from Mouse.

Quick Overview for CD79b antibody (C-Term) (ABIN6936828)

Target: CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD79b antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: B29-123

Product Details anti-CD79b Antibody

Binding Specificity: C-Term

Specificity: CD79 (also designated Ig chains, designated CD79B or B29. The B cell antigen receptor complex (BCR) is formed by the association of CD79 with a membrane immunoglobulin, such as IgM or IgD. The membrane immunoglobulins IgM and IgD achieve surface expression and antigen presentation function in response to CD79 association. The cytoplasmic tails of both CD79A and CD79B contain an ITAM (immuno-receptor tyrosine-based activation) motif, which acts to initiate the BCR signaling reactions by binding to and activating tyrosine kinases.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide representing the c-terminus from residue 215 of the murine B29 polypeptide.

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Daudi or Ramos cells. Germinal center B- cells in a lymph node or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD79b

Target: CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))

Alternative Name: CD79B

Background: AGM6, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig-beta, IGB, Immunoglobulin-associated B29 protein,CD79b (B-Cell Marker)
Cellular localisation: Cell surface

Molecular Weight: 39kDa

Gene ID: 974, 89575

UniProt: P40259

Pathways: BCR Signaling