CDK1 antibody (C-Term)

Catalog No. ABIN6936870

The Mouse Monoclonal anti-CDK1 antibody has been validated for WB, IHC, IF, IP, IHC (f), Act and KAA. It is suitable to detect CDK1 in samples from Xenopus laevis.

Quick Overview for CDK1 antibody (C-Term) (ABIN6936870)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Activation (Act), Kinase Activity Assay (KAA)

Clone: A17-1-1

Product Details anti-CDK1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes a 34 kDa protein (cdk1), identified as p34cdc2 (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of the protein and its core is thought to be LGTPNNEV (aa220-227 in murine cdc2). It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This MAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.

Cross-Reactivity (Details): Human. Mouse. Rat. Guinea Pig. Woodchuck. Xenopus. Chicken.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Inhibits activation of p34cdc2 kinase by cyclins,Immunofluorescence (1-2 μg/mL), Immunoprecipitation, Kinase Assay, Western Blot, ,Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human tonsil (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDC2

Background: Cdc2, CDC28A, CDK1, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cyclin-dependent kinase 1, p34 Protein Kinase, P34CDC2,Cdk1 / p34cdc2 Serine-Threonine Kinase
Cellular localisation: Nuclear and Cytoplasmic

Molecular Weight: 34kDa

Gene ID: 983, 334562

UniProt: P06493

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA