CDC20 antibody (Cell Division Cycle 20 Homolog (S. Cerevisiae))

Details for Product anti-CDC20 Antibody No. ABIN6936871
Antigen
  • X-FZY
  • ba276h19.3
  • cdc20a
  • fizzy1
  • p55cdc
  • CDC20A
  • bA276H19.3
  • p55CDC
  • 2310042N09Rik
  • C87100
  • cell division cycle 20
  • cell division cycle 20 L homeolog
  • cell division cycle 20 homolog (S. cerevisiae)
  • cdc20
  • CDC20
  • cdc20.L
  • Cdc20
Reactivity
Human
167
54
49
8
4
4
4
3
3
3
1
1
1
1
Host
Mouse
98
72
Clonality (Clone)
Monoclonal ()
Conjugate
This CDC20 antibody is un-conjugated
7
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
85
77
68
44
42
40
24
8
3
3
2
Options
Immunogen Urea-denatured His6 Cdc20 human recombinant protein
Clone AR12
Isotype IgG1, kappa
Purification Purified by Protein A/G
Plasmids, Primers & others Plasmids, Primers & others CDC20 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CDC20 (CDC20 Antibody Abstract)
Background Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
Molecular Weight 55kDa
Gene ID 991
UniProt Q12834
Application Notes

Positive Control: Ramos or HeLa cells. Tonsil or gastric carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions For Research Use only
Buffer 10 mM PBS without BSA and without Azide.
Preservative Azide free
Storage 4 °C,-80 °C
Storage Comment Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
Expiry Date 24 months
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