N-Cadherin antibody (Extracellular Domain)

Catalog No. ABIN6936893

This Mouse Monoclonal antibody specifically detects N-Cadherin in WB, IHC, IF, FACS and IHC (f). It exhibits reactivity toward Human.

Quick Overview for N-Cadherin antibody (Extracellular Domain) (ABIN6936893)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 8C11

Product Details anti-N-Cadherin Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Cross-Reactivity (Details): Human and Mouse. Others not known.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human N-cadherin extracellular domain

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blotting (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tis with1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HUVEC cells. Human heart, pancreas or cerebral cortex (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CDH2

Background: Cadherin-2 N cadherin neuronal, Cadherin-2 type 1, Cadherin-2, Calcium dependent adhesion protein neuronal, CD325, CDH2, CDHN, CDw325, N-Cadherin, NCAD,N-Cadherin / Cadherin-2 / CD325 (NCAD)
Cellular localisation: Cell surface

Molecular Weight: 130-140kDa

Gene ID: 1000, 464829

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane