CNN1 antibody
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- Target See all CNN1 Antibodies
- CNN1 (Calponin 1 (CNN1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CNN1 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
- Cross-Reactivity (Details)
- Human. Rat.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Crude human uterus extract
- Clone
- CALP
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CNN1 Primary Antibody
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- Application Notes
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: K562 cells, Myoepithelial cells in breast ducts.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- CNN1 (Calponin 1 (CNN1))
- Alternative Name
- CNN1 (CNN1 Products)
- Synonyms
- CN antibody, CnnI antibody, SMCC antibody, Sm-Calp antibody, XclpH3 antibody, clpH3 antibody, cnn2 antibody, calponin 1 antibody, calponin 1, basic, smooth muscle L homeolog antibody, cnn1 antibody, Cnn1 antibody, CNN1 antibody, cnn1.L antibody
- Background
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Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC,Calponin-1 (Smooth Muscle Marker)
Cellular localisation: Cytoplasmic - Molecular Weight
- 34kDa
- Gene ID
- 1264, 465929
- UniProt
- P51911
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