COL7A1 antibody

Catalog No. ABIN6937011

The Mouse Monoclonal anti-COL7A1 antibody is suitable to detect COL7A1 in samples from Human. It has been validated for WB, IF, ELISA, FACS, Coat and IHC (f).

Quick Overview for COL7A1 antibody (ABIN6937011)

Target: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This COL7A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: LH7-2

Product Details anti-COL7A1 Antibody

Specificity: Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Cross-Reactivity (Details): Human. Sheep. Goat. Horse. Guinea Pig. Cow.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Collagen VII protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for COL7A1

Target: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Alternative Name: COL7A1

Background: Alpha 1 type VII collagen, Collagen 7, Collagen alpha 1(VII) chain, Collagen type VII alpha 1, Collagen VII alpha 1 polypeptide Col7a1, EBD1, EBDCT, EBR1, Long-chain (LC) collagen,Collagen VII
Cellular localisation: Secreted. Extracellular matrix. Basement Membrane.

Molecular Weight: 290kDa

Gene ID: 1294, 476218

UniProt: Q02388