CPA1 antibody

Catalog No. ABIN6937013

This Mouse Monoclonal antibody specifically detects CPA1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for CPA1 antibody (ABIN6937013)

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CPA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CPA1-2711

Product Details anti-CPA1 Antibody

Purpose: Carboxypeptidase A1 / CPA1 (Pancreatic Cancer Marker) Antibody

Specificity: Human pancreatic procarboxypeptidase A exists as three different active forms, two of which are designated carboxypeptidase A1 (CPA1) and carboxypeptidase A2 (CPA2). CPA1, also known as CPA, is a 419 amino acid secreted monomeric protein that is highly expressed in pancreatic tissue. Functioning as a pancreatic exopeptidase, CPA1 uses zinc as a cofactor to catalyze the release of C-terminal amino acids from a variety of proteins, thereby playing a key role in protein digestion and degradation. Via its catalytic activity, CPA1 is also thought to be involved in zymogen (proenzyme) inhibition, probably functioning to block enzyme activation pathways. Abnormal levels of CPA1 are associated with pancreatic cancer, suggesting a possible role in either tumor progression or tumor suppression events.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant full-length human CPA1 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Pancreas. Pancreas tissue lysate.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CPA1

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Alternative Name: CPA1

Background:

Carboxypeptidase A1 (pancreatic), CPA1, Pancreatic Carboxypeptidase A1, Procarboxypeptidase A1 pancreatic

Pancreas. Pancreas tissue lysate.

Molecular Weight: 47kDa

Gene ID: 1357, 2879

UniProt: P15085