CPS1 antibody
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- Target See all CPS1 Antibodies
- CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CPS1 antibody is un-conjugated
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Application
- Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- This MAb recognizes a protein of 165kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells.Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC's occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.
- Cross-Reactivity (Details)
- Human. Dog.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human CPS1 protein
- Clone
- SPM615
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CPS1 Primary Antibody
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- Application Notes
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Known_Application: Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Liver or Hepatocellular Carcinoma (HCC).
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))
- Alternative Name
- CPS1 (CPS1 Products)
- Synonyms
- CPSASE1 antibody, cps antibody, 4732433M03Rik antibody, CPS antibody, D1Ucla3 antibody, Cps1 antibody, carbamoyl-phosphate synthase 1 antibody, carbamoyl-phosphate synthase 1 L homeolog antibody, carbamoyl-phosphate synthetase 1 antibody, peptidase M20 domain containing 1 antibody, CPS1 antibody, Cps1 antibody, cps1.L antibody, cps1 antibody, PM20D1 antibody
- Background
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Carbamoyl-phosphate synthetase 1 (CPS1), Carbamoylphosphate synthetase 1, CPSase 1, CPSASE1,CPS1 / Carbamoyl-Phosphate Synthetase (Hepatocellular Marker)
Cellular localisation: Finely granular cytoplasmic - Molecular Weight
- ~165kDa
- Gene ID
- 1373, 149252
- UniProt
- P31327
- Pathways
- Response to Growth Hormone Stimulus, Cellular Glucan Metabolic Process
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