CD35 antibody

Catalog No. ABIN6937019

This Mouse Monoclonal antibody specifically detects CD35 in FACS, IF and IHC (f). It exhibits reactivity toward Human.

Quick Overview for CD35 antibody (ABIN6937019)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: E11

Product Details anti-CD35 Antibody

Specificity: Recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This MAb is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein.Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

Cross-Reactivity (Details): Human. Baboon. Cynomolgus Monkey. Rhesus Monkey.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Intact human monocytes

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Follicular dendritic cells (FDC) in tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CR1

Background: C3 binding protein, C3b/C4b receptor, C3BR, C4BR, Complement Component (3b/4b) receptor 1 including Knops blood group system, Complement receptor type 1, KN, Knops blood group antigen,CD35 / CR1 (Follicular Dendritic Cell Marker)
Cellular localisation: Cell surface

Molecular Weight: 210-220kDa

Gene ID: 1378, 334019

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity