Recombinant CD21 antibody (AA 44-196)

Catalog No. ABIN6937023

The Mouse Monoclonal anti-CD21 antibody is suitable to detect CD21 in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant CD21 antibody (AA 44-196) (ABIN6937023)

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD21 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RCR2-1952

Product Details anti-CD21 Antibody

Binding Specificity: AA 44-196

Purpose: Recombinant CD21 (Mature B-Cell & Follicular Dendritic Cell Marker) Antibody

Specificity: Recognizes a protein of 140 kDa, which is identified as the complement receptor 2(CR2)/CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro.CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha.CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 44-196) of human CR2 (CD21) protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: WEHI-231 cells. Human tonsil (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CR2

Background:

CD21, Complement C3d receptor (C3DR), Complement Receptor type 2 (CR2), CVID7, EBV receptor, EBV-R, Epstein-Barr virus receptor, EVBR, SLEB9

WEHI-231 cells. Human tonsil (IHC).

Molecular Weight: 140kDa

Gene ID: 1380, 445757

UniProt: P20023

Pathways: Complement System