CRYAB antibody

Catalog No. ABIN6937029

The Mouse Monoclonal anti-CRYAB antibody has been validated for WB and IHC (f). It is suitable to detect CRYAB in samples from Human.

Quick Overview for CRYAB antibody (ABIN6937029)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CPTC-CRYAB-1

Product Details anti-CRYAB Antibody

Specificity: Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into B-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.

Cross-Reactivity (Details): Human. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length CRYAB protein

Isotype: IgG2c kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T whole cell lysates, heart and brain.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: CRYAB

Background: AACRYA, Alpha B crystallin, Alpha-crystallin B chain, CRYA2, CTTP2, Heat shock 20 kD like protein, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY REN 27, Rosenthal fiber component,Crystallin Alpha B
Cellular localisation: Cytoplasm, translocates to nucleus during heat shock and resides in nuclear splicing speckles.

Molecular Weight: Predicted: 20kDa, Observed: 22-30kDa

Gene ID: 1410, 53454

UniProt: P02511