GM-CSF antibody

Catalog No. ABIN6937030

This anti-GM-CSF antibody is a Rat Monoclonal antibody detecting GM-CSF in WB, IP, FACS, Neut, IF and IHC (fro). Suitable for Human.

Quick Overview for GM-CSF antibody (ABIN6937030)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Neutralization (Neut), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BVD2-21C11

Product Details anti-GM-CSF Antibody

Specificity: Granulocyte/macrophage - Colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

Cross-Reactivity (Details): Human. Cynomolgus. Rhesus Monkey.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human GM-CSF protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Neutralization Studies (Order Ab without Azide), Immunoprecipitation (0.5-1 μg/500 μg protein lysate), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Frozen) (1-2 μg/mL for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Positive_Control: Lymph node or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: CSF2

Background: Burst Promoting Activity, Eosinophil Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sargramostim,GM-CSF (Granulocyte/Macrophage - Colony Stimulating Factor)
Cellular localisation: Secreted (extracellular)

Molecular Weight: 22kDa

Gene ID: 1437, 1349

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin