Cathepsin D antibody (AA 104-250)

Catalog No. ABIN6937058

This Mouse Monoclonal antibody specifically detects Cathepsin D in WB, IF and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Cathepsin D antibody (AA 104-250) (ABIN6937058)

Target: Cathepsin D (CTSD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cathepsin D antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CTSD-3276

Product Details anti-Cathepsin D Antibody

Binding Specificity: AA 104-250

Specificity: Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins. It is synthesized as an inactive 52kDa preprocathepsin D that is cleaved and glycosylated to form a 48kDa procathepsin D and then further cleaved to produce 34kDa and 14kDa subunits (heavy and light chains, respectively). Cathepsin D exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human Cathepsin D protein (around aa 104-250) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, K-562 or SK-BR3 cells. Human kidney, liver, lung or breast tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Cathepsin D

Target: Cathepsin D (CTSD)

Alternative Name: CTSD

Background: CatD, Cathepsin D, Ceroid lipofuscinosis neuronal 10, CLN10, CPSD, Epididymis secretory sperm binding protein Li 130P, Lysosomal aspartyl peptidase, Lysosomal aspartyl protease,Cathepsin D (Tumor Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 48kDa (Procathepsin D), 34kDa (Cathepsin D), 14kDa (Cathepsin D)

Gene ID: 1509, 654447

UniProt: P07339

Pathways: Peptide Hormone Metabolism